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healthy male dermal fibroblasts reprocellusa  (ReproCELL)

 
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    ReproCELL healthy male dermal fibroblasts reprocellusa
    Healthy Male Dermal Fibroblasts Reprocellusa, supplied by ReproCELL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/healthy+male+dermal+fibroblasts/healthy+male+dermal+fibroblasts+reprocellusa/pmc10907695__41467_2024_46343_MOESM2_ESM-50-0-14
    Average 90 stars, based on 1 article reviews
    healthy male dermal fibroblasts reprocellusa - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Differentiation of natural killer cells from induced pluripotent stem cells under defined, serum- and feeder-free conditions.
    Article Snippet: Background aims: Traditionally, natural killer (NK) cells are sourced from the peripheral blood of donors¡a laborious and highly donor-specific process.. Processes for generating NK cells from induced pluripotent stem cells (iPSCs) have demonstrated that it is possible to successfully generate renewable alloreactive NK cells that are not only functional in vivo but can also be genetically engineered for enhanced function.. However, poor standardization and cumbersome differentiation procedures suggest that further improvements in the control of the differentiation process are necessary.

    Generated:

    Article Title: synNotch-programmed iPSC-derived NK cells usurp TIGIT and CD73 activities for glioblastoma therapy
    Article Snippet: .. The hiPSC01 iPS cell line was generated by us at the Purdue Institute for Integrative Neuroscience Cell Engineering core facility. hiPSC01 cell identity was confirmed by STR analysis.Healthy male dermal fibroblasts (ReprocellUSA) were reprogrammed using the StemRNA 3 rd Generation Reprogramming kit (Reprocell USA) according to the manufacturer’s protocol. .. STR analysis for iPS cell lines has been shown for this line . iPSCs were maintained and passaged in mTesR TM 1 (StemCell Technologies, 85850) feeder-free maintenance medium on hESC-Qualified Matrigel (Corning, 354277) coated 6-well plates.



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    Direct reprogramming of somatic cells into hiNPSCs. a Direct generation of isogenic naive and primed hiPSCs. Fibroblasts were transduced with 3 Sendai viruses expressing a polycistron KLF4/OCT4/SOX2, MYC and KLF4 at a ratio of 5:5:3, respectively. Cells were split on feeders at day 7, and placed in the indicated media at day 9. Scale bar = 100 µm. b Summary of lines generated for this study in primed (KSR+FGF2, yellow) or naive culture media (RSeT, blue or T2iLGö, pink) originated from 5 different donors. c Different pluripotent states are induced depending on culture media. Transcriptomes of hiPSCs and hiNPSCs, control primed hESC lines H1 and H9 or the naive hESC line HNES1 were analyzed by PCA. Symbols represent donor lines, and size of the symbols represents the passage. Arrows have been drawn to highlight the reprogramming trajectories. d T2iLGö hiNPSCs are the closest to human epiblast cells. PCA of single-cell RNA-seq data sets from preimplantation embryo samples , compared to primed hPSCs from ref. and to primed/naive hiPSCs/hESCs from this study

    Journal: Nature Communications

    Article Title: Parallel derivation of isogenic human primed and naive induced pluripotent stem cells

    doi: 10.1038/s41467-017-02107-w

    Figure Lengend Snippet: Direct reprogramming of somatic cells into hiNPSCs. a Direct generation of isogenic naive and primed hiPSCs. Fibroblasts were transduced with 3 Sendai viruses expressing a polycistron KLF4/OCT4/SOX2, MYC and KLF4 at a ratio of 5:5:3, respectively. Cells were split on feeders at day 7, and placed in the indicated media at day 9. Scale bar = 100 µm. b Summary of lines generated for this study in primed (KSR+FGF2, yellow) or naive culture media (RSeT, blue or T2iLGö, pink) originated from 5 different donors. c Different pluripotent states are induced depending on culture media. Transcriptomes of hiPSCs and hiNPSCs, control primed hESC lines H1 and H9 or the naive hESC line HNES1 were analyzed by PCA. Symbols represent donor lines, and size of the symbols represents the passage. Arrows have been drawn to highlight the reprogramming trajectories. d T2iLGö hiNPSCs are the closest to human epiblast cells. PCA of single-cell RNA-seq data sets from preimplantation embryo samples , compared to primed hPSCs from ref. and to primed/naive hiPSCs/hESCs from this study

    Article Snippet: Primed hiPSCs from L7 human adult dermal fibroblasts extracted from a healthy male aged 51 years (Lonza cat. no. CC-2511 Lot 0000293971) were also used in this study. hESC lines H1 (WA01 Lot WB0111) and H9 (WA09 Lot WB0090) were obtained from the WiCell Research Institute, under authorization RE13-004 from the French embryo research oversight committee, Agence de la Biomédecine.

    Techniques: Transduction, Expressing, Generated, RNA Sequencing Assay